Coding

Part:BBa_K2586002:Design

Designed by: Robert Warneke, Janek Mei�ner   Group: iGEM18_Goettingen   (2018-09-10)


GltP: glutamate and glyphosate uptake transporter in Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1074
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 709


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.

Source

This basic part consists of parts derived from PCR amplification of wild type B. subtilis chromosomal DNA (strain B. subtilis 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).

References